Aneet Chandra, 24, entered the not guilty plea in King County Superior Court.
The Washington State Patrol says Chandra was behind the wheel of a car that was headed south on I-5 at about 11:50 p.m. on May 1. The car, with six passengers inside, crashed into the front of the barrier at the 6th Avenue off-ramp, then hit the right side barrier.
Witnesses said the car was going about 80 mph at the time of the May 1 crash.
A 20-year-old woman sitting on someone's lap in the back seat was ejected and killed. Everyone else in the car was hurt, and police reported smelling alcohol on the driver's breath.
Chandra's defense attorney asked the judge not to let TV cameras show his face at Wednesday's arraignment. The judge rejected the request and let the accused man's face be shown.
Chandra remains behind bars on $1 million bail.