The Grammy-winning R&B singer will perform the national anthem before the NFL championship game on Feb. 3 in New Orleans.
The league said Friday that Keys' three performances at the Super Bowl will be the most in the event's history. She sang "America the Beautiful" before the 2005 game, and was part of the pregame show in 2008.
Keys, who turns 32 next week, released her fifth studio album, "Girl on Fire," late last year.
The Super Bowl teams will be determined Sunday, when the San Francisco 49ers play the Atlanta Falcons in the NFC championship game, and the Baltimore Ravens face the New England Patriots in the AFC championship game.
The NFL said in October that Beyonce will be the star of the halftime show at this year's Super Bowl. She sang the national anthem at the 2004 NFL title game in her hometown of Houston.
Others who have performed the national anthem at past Super Bowls include "American Idol" winner Kelly Clarkson last year, as well as Whitney Houston, Billy Joel, Diana Ross, Neil Diamond and Mariah Carey.
At the 2011 Super Bowl between the Green Bay Packers and Pittsburgh Steelers in Dallas, pop star Christina Aguilera flubbed a line while belting out the national anthem. When she was supposed to sing the words "O'er the ramparts we watched, were so gallantly streaming," Aguilera instead repeated an earlier line, with a slight variation - drawing plenty of attention on Twitter.